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Short-term sleep deprivation leads to decreased systemic redox metabolites and altered epigenetic status

(3) carrying out gradient elution by taking the reversed-phase silica gel filler as the stationary phase, a saline solution with a pH value adjusted to 2-3 by phosphoric acid as the mobile phase Aand a mixed solution of acetonitrile and isopropanol as the mobile phase B, collecting fractions, and carrying out rotary evaporation to remove a part of the solvents so as to obtain a secondarily-purified solution

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